Salinity Tolerance in Free Living Plant Growth Promoting Rhizobacteria

Osmolytes, PGPR, Proline, Reducing Sugars, Total soluble sugars, Rhizobacteria ABSTRACT Free living rhizospheric bacteria from wheat rhizosphere have capability to produced plant growth promoting attributes under saline condition. The electrical conductivity (ECe) of saline soil samples from four different districts viz. Varanasi, Mau, Ballia and Ghazipur of Uttar PradeshIndia was 10.47 to 5.69 dSm and maximum bacterial population (6.9 X 10 cfu g soil) was found on NA media as compared to rest of the media like King's B, Jensen's N free, TSA and SEA. About 33% of bacterial isolates were survive up to more then 8% NaCl (w/v) and only 19% showed PGP attributes at higher NaCl concentration. Ten identified PGPRs were used in the present study for their osmotolerence mechanism. Proline content was increased with NaCl stress and maximum production was recorded with Isolate SU82.73 and 11.95 g mg protein at 0% and 10% NaCl (w/v) respectively. The tendency RS and TSS production in rhizobacterial isolates were reverse proportional to the salt (NaCl) concentration. and derived genera were dominant under high saline condition along with PGP attributes, which could be mitigate salinity levels and improve agriculture crops under saline condition.